Format for Seed Registration Protocol

1.	Crop Name	- Sunflower
2.	Season/region/time	- Rainy, monsoon/ major production area of sunflower
3.	Check variety	- Registered varieties
4.	Plot size	- 15 ft x 4 rows
5.	Spacing	- 2 ft x 9 in
6.	Design	- RCB design, replication 4-6 (depend on no of variety)
7.	Fertilizer rate
	Basal	- Compound    - 2 bags, Urea- 112 lb/ac, Potash- 56 Lb/ac,  T-super- 56 Lb/ac

8.  Care & Management    
-    Deeply plough & harrowing
-    Thinning one plant 15 days after sowing
-    Cleaning weed when plants are young
-    If pest and disease infected, control with suitable pesticide in time.
-    At the budding time, …………. not for logging
-    Irrigate to field when water is needed at the 75 % budding and flowering time.
-    For reduce postharvest losses, harvest in time, systematic threshing and drying under the sun. 
9.    Recorded data  
50 % germination date
-    Shall be recorded the day of 50% germination among all plants at small plot.                                                                                                                                                                   Flowing date
-    Shall be recorded the first day of flowers are blooms at small plot.
50 % flowering date
-    Shall be recorded the day of 50 % flowering among all plants at small plot. 
Plant height (cm)
-    Shall be recorded from plant base to leaf tip for 10 sample plants that can represent the whole research field.
Numbers of fill grain per head
-    Shall be counted fill grain form the flower of 10 sample plants. 
Head diameter (inches)
-    Shall be measured the flower from 10 sample plants.
Yield from 10 plants (g)
-    Shall be measured seed weight from 10 sample plants.
Plot yield (g)
-    Shall be measured seed weight from the small plot. 
1000 grain weight (g)
-    Shall be measured 1000 grain weight from the yield of 10 sample plants.
Oil percent 
-    Shall be tested oil content percent at lab.
Harvesting date
-    Shall be harvested when receptacle is turn straw color.
10. Calculating yield
                          Yield from sample area (g) x 43560
Yield        =-----------------------------------------------------------------
                          Sample size x 1000 x 32

Characteristic

1.	Parent name or line numbe
2.	Origin
3.	Testing date
4.	Testing season
5.	Testing region
6.	Check varieties
7.	Plant height
8.	Head diameter
9.	1000 grain weight (g)
10.	Oil content %
11.	Yield (bsk/ac)
12.	Duration
13.	Adaptable region
14.	Distinct character
15.	GMO crop or not